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KMID : 0613820160260010075
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Journal of Life Science
2016 Volume.26 No. 1 p.75 ~ p.82
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Identification and Molecular Characterization of Superoxide Dismutase Genes in Pseudomonas rhodesiae KK1 Capable of Polycyclic Aromatic Hydrocarbon Degradation
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Lee Dong-Heon
Oh Kye-Heon
Kim Seung-Il
Kahng Hyung-Yeel
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Abstract
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Pseudomonas rhodesiae KK1 has been reported to degrade polycyclic aromatic hydrocarbons (PAHs), such as anthracene, naphthalene, and phenanthrene, which are considered major environmental contaminants. Interestingly, antioxidant genes, including superoxide dismutase, are known to be expressed at different levels in response to environmental contaminants. This study was performed to identify the superoxide dismutase gene in strain KK1, which may be indirectly involved with degradation of PAHs, as well as to investigate the expression pattern of the superoxide dismutase gene in cells grown on different PAHs. Two types of superoxide dismutase genes responsible for the antioxidant defense mechanism, Mn-superoxide dismutase (sodA) and Fe-superoxide dismutase (sodB), were identified in P. rhodesiae KK1. The sodA gene in strain KK1 shared 95% similarity, based on 141 amino acids, with the Mn-sod of P. fluorescens Pf-5. The sodB strain, based on 135 amino acids, shared 99% similarity with the Fe-sod of P. fluorescens Pf-5. Southern hybridization using the sod gene fragment as a probe showed that at least two copies of superoxide dismutase genes exist in strain KK1. RT-PCR analysis revealed that the sodA and sodB genes were more strongly expressed in response to naphthalene and phenanthrene than to anthracene. Interestingly, sodA and sodB activities were revealed to be maintained in cells grown on all of the tested substrates, including glucose.
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KEYWORD
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Antioxidant enzyme, PAHs, Pseudomonas rhodesiae KK1, RT-PCR, SOD
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